Growing Mushrooms from a Spore Syringe
Posted by Mushbox Admin on 1/11/2011
to What you need to know
There is several methods for growing mushrooms and most of them start by using a spore syringe, a liquid culture, or live spawn transfer.
The most common method is to use a multi-spore syringe. This is mushroom spores mixed with water inside a syringe ready for injection. If you need to learn more about how spores are formed and how mushrooms reproduce click here.
This spore syringe method is reliable and effective but using a liquid culture or live spawn is much faster as the mycelium is already active. When you start with spores the spores need to take time to germinate and come to life, then start colonizing the substrate and form into mycelium. So if you have it available it is best to start with an already live liquid culture or spawn. Click to learn more about liquid culture and live spawn.
Using a spore syringe to inoculate a substrate
You can get a spore syringe by purchasing one online from a reliable vendor like Spores101.com ( enter code shroomtalk for 5$ off ). Once you have the spores syringe you will also need to acquire a substrate that works with the mushroom you want to grow. You can view a variety of substrates here. Be sure to select a pre-sterlized substrate as that is what is needed to work with spores. The substrates that are not already sterilized are meant to be used with a casing technique later on.
A good substrate that works with almost every type of spores is the Compost Substrate Bags. This reliable form of substrate has a small patch of rye grain berries that will allow the spores to take hold faster, then move onto colonizing the compost.
Video on using the Compost Bags
Once you have inoculated the substrate you need to incubate it until it is fully colonized. This can take between 1-3 weeks, and even sometimes longer depending on the species of mushroom growing and the environment conditions. You want it to be warm and dark maintaining the incubation temps required for the species of mushroom you want to grow, usually between 79-82D. F.
However you also need to account for the natural heating that the colonizing mycelium creates, it can produce a degree or more of heat while consuming the substrate. To much heat can stunt growth and cause contamination, to little heat can also stunt growth but won't cause as much damage, it will just slow down and even stop colonizing if it gets to cold. This is a good method for mycelium storage, leaving it in the fridge.
You should check the incubation status often and see the growth, you should start to see areas of white forming around the main injection points within 10 days of inoculation. After that it will spread consistantly and should gather speed as it gets larger. You can see the mycelium reaching out as it consumes the substrate and taking up any space. Soon it will connect with itself and form a solid mass of mycelium or live spawn.
Once the myceium has fully colonized the entire mass of the substrate you can move onto the next step. From there you can fruit the mushrooms directly inside the bag or break up the mycelium and use it with a casing layer to grow even more mushrooms that are much larger, and get multiple flushes.
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Date 4/1/2012 7:10:00 PM
mike
101 on the way my order stopped by you for substrate??
Date 4/29/2012 10:01:00 AM
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Date 2/2/2013
charles h. conners
looking for info for mushroom growing for the maket
Date 4/3/2013
crumpets
add a link to purchase the bag.
Date 6/18/2013
Sheets
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Date 9/1/2013
hunter stone
just got my kit yesturday very pleased if i get anything else its goona be from this mushbox fosho.
Date 12/7/2013
waterson
what is the yield of one 10 ml syringe ?
Date 1/4/2014
Jack beard
If I have my spore syringe and I purchase the bird seed bag and mushbox from you will that be sufficient on my supplies I need to grow? I've been reading here for some time now and have decided to make my first purchase if you could respond that'd be great.
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 3/25/2014
chandler olson
Yo i just got a golden teacher syringe and do i shoot it up some cow shit or bird seed? idk wtf to do with this lmao i just need some legit help first time grower and the simplest way idc how long as long as its the easiest way and ill be straight. HELP ME HELP ME HELP ME!!!
Date 7/23/2014
durkalizer
I will help u guys out if need be... just contact me by email... Though I will charge for my services since you are too lazy to just look up the information yourself... I ordered Equadorian from spores101.com for my first time grow and used 1-2 lbs of dry horse manure to procure a lil under half a lb of donkey dick sized mushrooms... [email protected]
Date 3/5/2016
Matthew Horn
Hello,I have a question for you can I use rice cakes for substrate and how much do I in inoculate the rice cakes
Date 1/11/2017
Floydlieno
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Date 5/6/2017
Jack
I ordered a spore syringe of golden teacher. I am planning on getting a plastic bin and filling it with fresh cow manure.. What steps do I take and should I just spray the spores on top of fresh maure, or spray and cover, If anyone can give me instructions when I get the manure and spores I would appreciate it. I live in a warm climate and plan to keep them outside. Thank you!!
Date 5/6/2017
Jack
I ordered a spore syringe of golden teacher. I am planning on getting a plastic bin and filling it with fresh cow manure.. What steps do I take and should I just spray the spores on top of fresh maure, or spray and cover, If anyone can give me instructions when I get the manure and spores I would appreciate it. I live in a warm climate and plan to keep them outside. Thank you!!
Date 2/12/2018
Austin
I have some syringes to grow but don't know what to start with I would like some pointers
Date 7/20/2018
Lawrence Gasior Jr
I haven't heard of cultivating mushrooms this way before. Doesn't sound right at all. You don't inject your spores into a bag with substrate and grain both. You must colonize grain first, then spawn to bulk substrates like coir and vermiculite. Then once that's fully colonized you can put it into fruiting conditions yada yada, 6000k lighting and plenty of fresh air exchange. Monotubs are a good way to grow mushrooms. This article is too vague and misinformative in my opinion.
Date 7/20/2018
Lawrence Gasior Jr
I haven't heard of cultivating mushrooms this way before. Doesn't sound right at all. You don't inject your spores into a bag with substrate and grain both. You must colonize grain first, then spawn to bulk substrates like coir and vermiculite. Then once that's fully colonized you can put it into fruiting conditions yada yada, 6000k lighting and plenty of fresh air exchange. Monotubs are a good way to grow mushrooms. This article is too vague and misinformative in my opinion.
Date 7/23/2018
Shay Rollins
Date 7/23/2018
Shay Rollins
First time grower need help with everything!!! I’m lost I have so many questions hit me back
Date 12/12/2018
luke Sherrell
I inoculated a all in one grow bag 4 days ago and have been keeping it in my bathroom closet..I used 5cc of a spore syringe..how long should it take for me to start seeing mycellium growth on the rye berries??..Thanks for any help
Date 9/5/2019
Julie Holt
As a first time grower, I guess I am impatient. Just making sure boom block is ok to use for growing GT and it has been almost 2 weeks and I see nothing. Just unsure if I did this correctly.
Date 4/3/2020
Shroom Shop
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Date 5/3/2022
Will
I got four different strains I spent about $800 for in syringes some albino very rare ones and regular easy A+ B+ I need to grow them immediately and I need the easiest way to go can anyone help me
